Abstract
Malaria is a serious public health problem worldwide despite concerted efforts to control and eliminate it. Zoonotic malaria is emerging as a big threat to malaria elimination efforts. There is a big knowledge gap in our understanding of zoonotic malaria. It is difficult to adequately detect and identify Plasmodium species responsible for zoonotic malaria with conventional diagnostic tools. Although molecular tools have good sensitivity and specificity to diagnose zoonotic malaria, they are not readily available in resource-limited settings. There is limited data on the epidemiology of zoonotic malaria in sub-Saharan Africa. However, various reports signal for the presence of its risk lurking in the area. Therefore, organized and comprehensive studies are needed to characterize its epidemiology in sub-Saharan Africa to guide strategies of mitigating zoonotic malaria in particular and malaria in general.
Introduction
Zoonotic pathogen is a microorganism that colonizes or infects animals and can be transmitted to humans through vector or via direct contact with the animal or its products. Zoonotic disease causation in humans, usually, is accidental and not primarily for evolutionary survival [1]. An interest in potentially zoonotic malaria started after accidental infection of laboratory workers with Plasmodium cynomolgi (P. cynomolgi) via an Anopheles mosquito in 1960 [2]. Recent scientific developments show the possibility of zoonotic malaria. Various studies have reported human infection by macaque and simian malaria parasites, mainly by Plasmodium knowlesi (P. knowlesi, P. simium) and Plasmodium brasilianum (P. brasilianum) [3-6]. P. knowlesi is common in Southeast Asia [3], whereas Plasmodium simium (P. simium) and P. brasilianum are common in Latin America [5,6]. However, we lack concrete evidence on whether all human cases originate from animals or human-to-human transmission is occurring [4]. This mini review focuses on the biology, clinical pictures, diagnosis, treatment and epidemiology of zoonotic malaria in sub-Saharan Africa, and its implication for malaria elimination.
Biology and Clinical Picture
According to various experimental studies, parasites of monkeys, such as P. cynomolgi, P. brasilianum, P. eylesi, P. inui, and P. simium have been shown to infect human via mosquitoes [7, 8]. Besides, isolation of the DNAs of the Plasmodium species commonly infecting human from African anthropoid apes alarm for possibility of zoonosis [9]. However, P. knowlesi is the widely accepted Plasmodium parasite of zoonotic malaria, particularly in Southeast Asian countries, and recently became the fifth human malaria parasite [10]. Monkeys are reservoir host of knowlesi malaria [9]. Furthermore, a recent molecular study in Africa revealed zoonotic origin of the human malaria parasite P. malariae, which is known to infect chimpanzees [9,11].
Due to close similarity between P. vivax, and P. cynomolgi, various experimental studies including culturing P. cynomolgi helped to understand the life-cycle of P. vivax [12,13]. Similar to P. vivax, P. cynomolgi produces dormant liver stage hypnozoite. It also favors infecting reticulocytes and have the Schuffner’s dot visible on infected erythrocyte membrane [14,15]. Zoonotic malaria exhibits similar symptoms of the known human malaria including myalgia, anorexia, fever, nausea and others. Human malaria caused by simian Plasmodium is often mild [9,14]. The clinical picture of malaria due to P. knowlesi infections is often uncomplicated. However, it can cause severe malaria in about 10% of patients in Malaysia [4,16,17]. The incubation period of knowlesi malaria lasts for 9-15 days [9]. The developmental cycle of P. knowlesi occurs every 24 h, and hence cause quotidian malaria in which patient fevers peak every day [7,9]. Similar to P. vivax, P. cynomolgi commonly causes asymptomatic malaria and produces dormant liver stage hypnozoite [14].
Transmission
Leucosphyrus group of Anopheline mosquitoes transmit P. knowlesi and the transmission is mainly zoonotic. This type of malaria is dominant malaria in Malaysia, where its transmission is restricted to jungle thereby putting humans entering jungle in transmission area at risk. It is quite common to see human cases of P. knowlesi in travelers returning from Southeast Asia [4].
A recent sequencing study has shown that P. simium, a malaria parasite of non-human primates (NHP), can cause zoonotic infection of human in Brazil. In fact, the study revealed that ability of the parasite to undergo genetic adaptations underscore its advantage to switch between host species [18]. An. vinckei, An. moucheti and An. marshallii serve as competent vectors of ape Laverania and non-Laverania parasites thereby mediating transmission of zoonotic malaria [19].
Management of Zoonotic Malaria
Diagnosis
Microscopic examination of Giemsa stained thick and thin blood film is the routine diagnosis for malaria of humans and monkeys. However, it is difficult to differentiate between simian Plasmodium and the known human Plasmodium species solely by morphological criteria. Besides, the capacity of an extensively used malaria RDTs to sufficiently characterize simian Plasmodium species is limited [9,20].
The development of molecular methods such as polymerase chain reaction (PCR) was instrumental in the knowledge gained on zoonotic malaria [10,14]. For long time, P. knowlesi has been misdiagnosed as P. malariae. Nevertheless, PCR is now the definitive method for differentiating P. knowlesi from P. malariae and other human malaria parasites [21]. Likewise, PCR helped to differentiate between P. cynomolgi and P. vivax (14). Nevertheless, due to low prevalence (in terms of detected cases); high cost and inadequate availability of PCR, it is conceivable that zoonotic malaria is under diagnosed [20]. Generally, highly sensitive and specific tools such as PCR are critical for accurate detection of both zoonotic and human-only Plasmodium species [22].
Treatment
Chloroquine is the drug of choice for zoonotic malaria treatment regardless of resistance to the drug by other species of Plasmodium. In the case of P. knowlesi infection, a chloroquine-primaquine combination therapy is recommended [9].
Epidemiology in Sub-Saharan Africa
Various reports indicate the circulation of primate-infecting Plasmodium species in the equatorial forest region of Africa [23,24]. Despite the lack of an organized and comprehensive study, there are many reports from human and monkeys in different countries signaling for the presence of zoonotic malaria risk revolving in the continent [24-27]. A molecular study targeting cytochrome b gene conducted by Duval et al. in Cameroon showed similarity of the Plasmodium species between human and chimpanzee in which a chimpanzee Plasmodium strain was found to be genetically identical to variant Plasmodium ovale (P. ovale) type [27]. A variant P. ovale species is detected from a patient in Ghana. This parasite is highly similar to a variant P. ovale detected from Chimpanzee in Cameroon [25].
There are no reports of confirmed P. knowlesi infection in sub-Saharan Africa. The possible explanation for this absence of zoonotic malaria in the region can be absence of the common reservoir hosts (the pig-tailed and the long-tailed monkeys) and the potential misdiagnosis [26].
Factors Determining Zoonotic Malaria
According to reports of recent studies, risk factors for infection with zoonotic malaria parasites include the male sex, contact with macaque monkeys, agricultural expansion, presence of mosquito vector, human behavior, travel to forest areas and forest fragmentation [28-31]. Generally, host factors, species of the Plasmodium, social factors such as migration, environmental factors such as dense jungle and vector factors are pivotal to the risk of zoonotic malaria [32,33].
Gaps
Data on zoonotic malaria epidemiology is limited, particularly in sub-Saharan Africa. This might be partly due to low prevalence or misdiagnosis. In co-endemic settings, misdiagnosis is expected since microscopic morphological appearance of P. cynomolgi and P. knowlesi is similar to P. vivax and P. malariae, respectively [20,22,34]. A recent analysis of the P. simium genome showed a close phylogenetic relationship between P. simium and P. vivax [18]. We have limited knowledge on zoonotic malaria that can guide designing effective intervention to combat and eliminate it [33].
The five Plasmodium species that infect humans have been found in non-human primates (NHPs). This means these NHPs can potentially serve as reservoirs for transmission of malaria to humans. Besides, given evolutionary process, Plasmodium species those infecting NHPs may eventually become causes for human malaria [35,36]. Based on molecular epidemiology studies, Laverania infected apes do not currently serve as a reservoir of human infection [37,38]. Nevertheless, in depth researches are critical to resolve these discrepancies and devise interventions.
Impact of Zoonotic Malaria on Elimination Efforts
Considering the recent malaria outbreaks of P. knowlesi in Southeast Asia and P. simium in Brazil, it is conceivable that intensified human intervention on the natural forest might increase the risk of zoonotic malaria [37]. The deletion of Duffy binding protein (DBP1) gene in P. simium may facilitate invasion of human red blood cells thereby facilitating zoonotic infection, which in turn sustains malaria transmission [18].
If we do not have clear knowledge on zoonotic malaria with its epidemiology in time and space, we might not be in better position to mitigate it. Moreover, it could eventually complicate efforts to eliminate malaria [1]. In the presence of continued human contact with the monkey reservoir and the presence of competent mosquito vectors, zoonotic malaria will not be eliminated by current intervention that mainly focused at human-only Plasmodium species [17]. Following decline in the prevalence of human-only malaria due to intensified elimination efforts, zoonotic malaria could become prominent public health problem in co-endemic settings. Besides, if areas approaching elimination use molecular surveillance, the previously misdiagnosed zoonotic malaria by conventional tools might persist and delay the achievement of elimination [34,39].
Conclusion and Recommendation
Zoonotic malaria is becoming public health problem by threatening malaria elimination efforts. There is a big knowledge gap in our understanding of zoonotic malaria. There is paucity of data on the epidemiology of zoonotic malaria in sub-Saharan Africa. Admirable advancements of molecular techniques with their promising application in epidemiology can fine-tune our understanding on zoonotic malaria. That knowledge will prompt discovery of mitigation tools and intervention strategies to mitigate zoonotic malaria in particular and keep us on the track to eradicate malaria in general.
A concerted effort is needed on basic science to understand the biology of simian Plasmodium species with zoonotic potential. An integrated control program including ecological approach is required to mitigate the eminent public health threat from zoonotic malaria. Considering zoonotic malaria into mitigation efforts is key to consolidate gains and advance towards malaria elimination.
Author Contributions
Author Contributions
Aklilu Alemayehu has conceived, made online search of relevant articles, and wrote the first draft. Then, he critically reviewed, wrote the final draft, read and approved it for this submission.
Funding
This research received no external funding.
Acronyms and Abbreviations
DBP: Duffy binding protein
NHP: Non-human primates
PCR: Polymerase Chain Reaction
RDT: Rapid Diagnostic Test
