¹Assistant Professor, Department of Plant Biology and Plant Biotechnology, Guru Nanak College, Chennai, Tamil Nadu, India.
²Scholar, Department of Plant Biology and Plant Biotechnology, Guru Nanak College, Chennai, Tamil Nadu, India.
Poonam Sethi, Assistant Professor, Department of Plant Biology and Plant Biotechnology, Guru Nanak College, Chennai, Tamil Nadu, India.
Poonam Sethi, et al. Efficacy of an Organic Formulation: Bijamrutham [Liquid Gold] on Seeds of Green Gram. J. Food Nutr. Vol 5, Iss 2. (2026). DOI: 10.58489/2836-2276/047
© 2026 Poonam Sethi. This is an open-access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Bijamrutham, Bordeaux mixture ,Green gram, Chemical spray, Sprouts.
The green gram sprouts were subjected to bijamrutham formulation and results demonstrated that, at a concentration of 100 μl showed promising effects, comparable to the commonly used Bordeaux mixture 10%. Furthermore, the organic spray also exhibited potential as a soil restorative agent, offering a sustainable alternative to chemical fertilizers with respect to seed vigour as calculated before and after treatment of bourdaeux mixture. Recommended for certain pulses and other vegetable crops controls disease and enhances seed growth hence a promising organic fertilizer. Zero Budget Natural Farming (ZBNF), is the need of the hour where only natural resources are used as fertilizers for multiple cropping systems recently practiced by thousands of farmers in India. However, without any scientific proof, this traditional and ancient technique is mocked as a bugged theory in the scientific community. In the current study, we have investigated the effect of Beejamrutham formulation—on seed vigour with analysis and the economics of ZBNF.
Medicinal plants are applied in the treatment of various plant diseases in India. The concepts of organic agriculture were developed in the early 1900s by Sir Albert Howard, F.H. King and Rudolf Steiner who believed that the use of animal manures (often made into compost), cover crops, crop rotation, and biologically based pest controls resulted in a better farming system. Howard, having worked in India as an agricultural researcher, gained much inspiration from thetraditional and sustainable farming practices he encountered there and advocated for their adoption in the West [1] Liquid organic formulations like bijamrutham prepared by fermentation process from locally available ingredients are rich sources of beneficial microflora which gives support and helps in stimulating plant growth and better vegetative growth by producing good quality yield. [2] This liquid gold is prepared from cow dung and cow urine and used for seed treatment before the process of sowing the seeds. It shows 100% better results when it is used on the day of preparation Sprouts of green gram (Phaseolus radiatus) an interesting and nutritious food. They have been used by the Chinese for centuries, and they are now widely accepted in our diet. The possibilities of enhancing their nutritional qualities through plant breeding are great. The study explored the efficacy of Bijamrutham, an organic preparation derived from cow dung,cow urine and jaggery, a solution in which the seeds were soaked. The results demonstrated that at a concentration of 100 μl showed promising effects, comparable to the commonly used Bordeaux mixture 10%. Furthermore, the organic spray also exhibited potential as a soil restorative agent, offering a sustainable alternative to chemical fertilizers. These findings highlight the importance of exploring organic solutions in agriculture and point towards the potential of organic fertilizer in improving tea plant health and yield while reducing the reliance on chemical controls.
Method of preparation of Bijamrutham [3]
1. 3 kg Indian cow dung is taken in a piece of cloth and sealed it by rope-pouch like
2. This sealed cloth is hung in the 10-litre water for 12 hours. 30 g of lime is added to the water for 12 hours.
4. The sealed pouch is squeezed and the cow dung is allowed to accumulate in that water.
5. 1 kg of farm soil is added to the solution and stirred well.
6. Ferment with3-liter Indian cow urine
7. Soak the seeds in this solution and then sow it
8. Applied in the early mornings for promising results
The ingredients are mixed as per the requirement in Table 1
|
Serial Number |
Ingredient |
Quantity |
|
1 |
Cow Dung |
3 kilograms |
|
2 |
Cow Urine |
3 Litres |
|
3 |
Farm Soil |
1 gram |
|
4 |
Lime |
30gram |
|
5 |
Water |
10 litres |
Table 1: Ingredients of Beejamrutham
This liquid gold rich in microbes hastens the natural functions of the soil there by increasing the growth rate of plants and their yield.. These microbes disintegrate the wastes in the soil there by making soil more fertile. Mainly used for treating seeds and also effective in young roots protection.
Experimental plant: Green Gram
The wild variety was procured from TNAU ( Tamil Nadu Agricultural University), Coimbatore.

Figure 1: Green Gram Seeds Used as Pulse
Calculation of Seed Vigor Index (Svi) Seed Vigor Index (SVI) was determined by following Williamson and Richardson's formula (1988) [4] Seed Vigor Index (SVI) = Germination percentage X Seedling Length (Root+Shoot) / 100 Root, Shoot and Seedling Length The normal seedlings were selected by random selection method after seven days of germination. The root, shoot length and total seedling length was measured and mean length was calculated after the application of Beejamrutham for 3 and 6-day intervals. Bourdeaux mixture 10% was the positive control [5] as widely applied by farmers of southern parts of India. Results The results demonstrated that bijamrutham, at a concentration of 100 μl showed promising effects, comparable to the commonly used Bordeaux mixture 10%. as a positive control. [Table 2].
|
Serial Number |
Concentrations of Bijamrutham formulation μl |
Sprouting percentage % |
Average Sprout length cm |
Bordeaux 10% on Average Sprouting percentage |
Bordeaux 10% on Average Sprout length cm |
||||
|
|
|
3 days |
6 days |
3 days |
6 days |
3 days |
6 days |
3 days |
6 days |
|
1 |
2 |
55 |
66 |
4.02 |
17.02 |
50 |
69 |
5.5 |
18.2 |
|
2 |
5 |
75 |
85 |
6.50 |
19.33 |
72 |
88 |
5.6 |
18.8 |
|
3 |
10 |
91 |
89 |
10.00 |
19.55 |
72 |
88 |
5.8 |
19.1 |
|
4 |
15 |
91 |
90 |
10.20 |
19.79 |
82 |
91 |
5.9 |
19.5 |
|
5 |
20 |
92 |
100 |
10.50 |
20.01 |
85 |
98 |
5.9 |
19.8 |
|
6 |
25 |
96 |
100 |
10.75 |
20.08 |
85 |
99 |
6.0 |
19.9 |
Table 2: Effect of Bijamrutham Formulation on Sprouting and Shoot Length Of Green Gram
These findings highlight the importance of exploring organic solutions in agriculture and point towards the potential of organic fertilizers in improving tea plant health and yield while reducing the reliance on chemical controls (Figures 2 and 3).

Figure 2: Preparation of Bijamrutham Formulation

Figure 3: Effect of Bijamrutham Formulation on Green Gram Sprouts
Seed vigour index was calculated and tabulated to prove the efficacy of the formulation on the various growth parameters of the pulse widely used as a sprout.
|
Serial Number |
Concentrations of Bijamrutham formulation μl |
Vigour Index for 3 days |
Vigour Index for 6 days |
Vigour Index Bordeaux 10% for 3 days |
Vigour Index Bordeaux 10% for 6days |
|
1 |
2 |
2.21 |
11.23 |
2.75 |
12.55 |
|
2 |
5 |
4.57 |
16.43 |
4.03 |
16.54 |
|
3 |
10 |
9.10 |
17.39 |
4.17 |
16.80 |
|
4 |
15 |
9.28 |
17.81 |
4.83 |
17.74 |
|
5 |
20 |
9.66 |
20.01 |
5.01 |
19.40 |
|
6 |
25 |
10.32 |
20.08 |
5.10 |
19.70 |
Table 3: Seed Vigour Index of Bijamrutham Formulation on Sprouting of Green Gram
Using bijamrutum a traditional formulation used widely in southern parts of India for pulses one of the traditional knowledge of ancient India. In earlier experiments it was proved that organic manures contain essential required nutrients which result in increased crop growth and yield [6] Very few references are available about scientific study of effect of cow dung on seed germination of important legume crops. Arachis hypogaea L. (Ground nut), Glycine max (L. )Merr. (Soybean), and Vigna radiata. (L.) R. Wilczek. (Green gram) Economically used for production of oil and pulses and are integral part as food in Maharashtra. This experiment was carried out to evaluate effectiveness of organic liquid treatments, including Bijamrita on seed germination and seedling growth of these legume crops.[7] Nitrogen-fixing bacteria and actinomycetes help in mobilising more plant nutrients and required plant growth promoting substances and other micronutrients are made available to the plants [8].Many environmental groups and governments have demanded decrease of inorganic fertilizer use in agriculture to diminish nutrient leaching into ground water or water pollution [9]. Similarly instead of applying chemical treatments to seeds organic liquid treatments must be advocated [10-11] and [12].