Research Article | DOI: https://doi.org/10.58489/2836-3590/016
*Corresponding Author: Apurvkumar M. Patel
Citation: Raj P. Raval, Apurvkumar M. Patel. (2024). Screening of Pearl millet germplasms against downy mildew disease. Pollution and Effects on Community Health. 3(1). DOI: 10.58489/2836-3590/016
Copyright: © 2024 Apurvkumar M. Patel, this is an open-access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Received: 21 August 2023 | Accepted: 15 February 2024 | Published: 05 February 2024
Keywords: Pearl millet, Downy mildew, Sclerospora graminicola, Germplasm.
Pearl millet or bajra [Pennisetum glaucum (L.) R. Br.] is one of the most important staple food for lakhs of poor people living in the semi-arid zones of India. Pearl millet is infected by a large number of diseases caused by fungal, bacterial, viral and nematode pathogens. Among these, downy mildew caused by Sclerospora graminicola (Sacc.) Schroet is the most destructive and widespread in India. Considering this, the present study on downy mildew of pearl millet was carried out screening of pearl millet germplasms under field condition against downy mildew disease. Total 95 pearl millet germplasms were evaluated under field conditions against downy mildew during kharif, 2017. Among 95 germplasms, 61 germplasms were found highly resistant, 22 germplasms were found resistant, 12 germplasms were found susceptible and none of germplasm was found highly susceptible.
Pearl millet is mainly grown in Gujarat, Rajasthan, Uttar Pradesh, Haryana and Maharashtra in kharif and rabi seasons (Anonymous, 2016a). While, in Gujarat, it is grown in 9.32 lakh hectares with a production of 10.97 lakh tonnes and productivity of 1177 kg/ha (GOG,2016).
The crop is nutritionally rich with a good balance of starch, protein and fat. Pearl millet provides 11 to 12 per cent of worlds’ supply of protein (Yadav
et al., 2011). The downy mildew pathogen was first reported on pearl millet in India in 1907 by E. J. Butler. However, during recent years the disease has been found to be very destructive and become a limiting factor for the cultivation of hybrid bajra (Sivaprakasan et al., 1975). symptoms were rarely seen as local lesions or isolated spots on leaf blade spots vary in shape and size and were at first chlorotic, produce sporangia and later became necrotic (Girard, 1975). The disease is affecting not only foliar parts, but also the ear head. In many affected plants, ears fail to form or if formed, they are malformed into green leafy structures; hence it is also known as the ‘green ear’ disease. Abandoning the crop in its premature stages is not quite uncommon due to the ravages of this disease (Sivaprakasan et al., 1975). Since the economic returns from this resource poor pearl millet crop are meagre; growing resistant varieties is the only alternative for combating this downy mildew disease. Therefore, it becomes necessary to screen the germplasms.
The experiment was conducted at Centre for Crop Improvement, Sardarkrushinagar Dantiwada Agricultural University, Sardarkrushinagar during Kharif, 2017 to study the screening of pearl millet germplasms against downy mildew in sick plot with higher oospore inoculums load. Each germplasm was sown in two replications. Recommended agronomic practices were adopted for successful raising the crop. Later on monitoring for downy mildew symptom development in the seedlings till the crop maturity was constantly examined. The total and downy mildew infected seedlings were counted to determine the disease incidence at 15 days interval. Irrigation was provided through sprinkler system frequently for maintaining high relative humidity (> 90 % relative humidity) to promote the infection and disease development. Downy mildew highly susceptible cultivar 7042S was used as infector rows (Inoculum donor) with a view to develop the maximum disease pressure. These rows were planted three weeks earlier than the test rows. The infector rows were sown uniformly throughout the entire length of the field. The test material of the respective trial was sown about three weeks after the sowing of infector rows. The indicator rows (susceptible genotype '7042S ' to assess the level of disease pressure) were planted along with the test rows at regular interval (after 10 rows).
mildew infestation from 1 to 5 rating scale as given below.
Observations recorded
In order to relate the productivity loss due to downy mildew severity, disease severity scores was taken on individual plant basis at crop maturity using a 1 to 5 scale.
Where,
1 | = | No infection, |
2 | = | 20 % productive tillers infected, |
3 | = | 50 % productive tillers infected, |
4 | = | 80 % productive tillers infected, and |
5 | = | All tillers infected or total plant killed. |
Disease scoring was carried on every 15 days interval up to crop maturity. The observed on per cent diseases index was worked out as described below (Anonymous, 2016b).
Where, n1, n2, n3, n4, and n5 are total number of plants in each of 1 to 5 rating class and N is the total number of plants in a plot. Observations on downy mildew incidence were recorded at 15 days interval up to crop maturity (90 days). The following formula was used to calculate per cent diseases incidence (Sharma et al., 2007).
Table – 1: Incidence of downy mildew disease in pearl millet germplasms
Sr. No. | Germplasms | Downy mildew incidence (%) | Mean Incidence(%) | Reaction | ||||
30 DAS | 45 DAS | 60 DAS | 75 DAS | 90 DAS | ||||
1 | 16976 | 3.57 | 3.57 | 5.36 | 5.36 | 5.36 | 4.64 | HR |
2 | 16110 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | HR |
3 | 42246 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | HR |
4 | 18037 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | HR |
5 | 15805 | 11.51 | 11.51 | 14.58 | 16.39 | 16.39 | 14.08 | S |
6 | 15078 | 6.52 | 6.52 | 8.70 | 8.70 | 8.70 | 7.83 | R |
7 | 22923 | 1.67 | 1.67 | 5.00 | 6.67 | 6.67 | 4.33 | HR |
8 | 1220 | 1.67 | 1.67 | 9.38 | 9.38 | 9.38 | 6.29 | R |
9 | 20229 | 0.00 | 0.00 | 1.67 | 1.67 | 1.67 | 1.00 | HR |
10 | 15787 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | HR |
11 | 1421 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | HR |
12 | 5972 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | HR |
13 | 1342 | 7.77 | 7.77 | 10.90 | 12.51 | 12.51 | 10.29 | S |
14 | 17840 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | HR |
15 | 18580 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | HR |
16 | 1225 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | HR |
17 | 22822 | 7.13 | 7.13 | 12.69 | 12.69 | 12.69 | 10.46 | S |
18 | 42182 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | HR |
19 | 42479 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | HR |
20 | 1202 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | HR |
21 | 1206 | 5.23 | 5.23 | 8.84 | 8.84 | 8.84 | 7.39 | R |
22 | 5969 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | HR |
23 | 15067 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | HR |
24 | 16010 | 0.00 | 3.85 | 3.85 | 5.77 | 5.77 | 3.85 | HR |
25 | 20437 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | HR |
26 | 15695 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | HR |
27 | 16625 | 3.23 | 3.23 | 4.84 | 4.84 | 4.84 | 4.19 | HR |
28 | 42299 | 5.56 | 5.56 | 9.26 | 9.26 | 9.26 | 7.78 | R |
29 | 15691 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | HR |
30 | 41530 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | HR |
31 | 15041 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | HR |
32 | 16745 | 3.70 | 3.70 | 5.56 | 7.41 | 7.41 | 5.56 | R |
33 | 16322 | 7.67 | 7.67 | 10.75 | 10.75 | 10.75 | 9.52 | R |
34 | 17130 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | HR |
35 | 17659 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | HR |
36 | 15924 | 14.58 | 14.58 | 18.75 | 18.75 | 18.75 | 17.08 | S |
37 | 15743 | 4.55 | 4.55 | 9.09 | 9.09 | 9.09 | 7.27 | R |
38 | 18839 | 10.26 | 10.26 | 14.56 | 14.56 | 14.56 | 12.84 | S |
39 | 15708 | 1.43 | 1.43 | 2.86 | 2.86 | 2.86 | 2.29 | HR |
40 | 16101 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | HR |
41 | 42852 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | HR |
42 | 17734 | 12.14 | 12.14 | 13.81 | 13.81 | 13.81 | 13.14 | S |
43 | 21334 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | HR |
44 | 16317 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | HR |
45 | 42078 | 4.69 | 6.25 | 6.25 | 7.81 | 7.81 | 6.56 | R |
46 | 18023 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | HR |
47 | 22559 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | HR |
48 | 17479 | 6.16 | 6.16 | 7.77 | 7.77 | 7.77 | 7.13 | R |
49 | 15738 | 9.38 | 9.38 | 12.50 | 12.50 | 12.50 | 11.25 | S |
50 | 22726 | 2.27 | 2.27 | 2.27 | 2.27 | 2.27 | 2.27 | HR |
51 | 41215 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | HR |
52 | 17928 | 00 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | HR |
53 | 17703 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | HR |
54 | 17933 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | HR |
55 | 17633 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | HR |
56 | 5984 | 3.84 | 3.84 | 7.68 | 7.68 | 7.68 | 6.14 | R |
57 | 6067 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | HR |
58 | 17179 | 6.57 | 6.57 | 9.86 | 9.86 | 9.86 | 8.55 | R |
59 | 22827 | 5.11 | 6.78 | 8.56 | 8.56 | 8.56 | 7.52 | R |
60 | 16128 | 3.49 | 3.49 | 3.49 | 5.41 | 5.41 | 4.25 | HR |
61 | 15001 | 11.04 | 11.04 | 12.60 | 14.21 | 14.21 | 12.62 | S |
62 | 42063 | 5.36 | 7.14 | 10.71 | 10.71 | 10.71 | 8.93 | R |
63 | 16060 | 4.08 | 4.08 | 8.25 | 8.25 | 8.25 | 6.58 | R |
64 | 15325 | 9.33 | 9.33 | 11.33 | 13.00 | 13.00 | 11.20 | S |
65 | 17604 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | HR |
66 | 6055 | 0.00 | 0.00 | 3.34 | 03.34 | 3.34 | 0.67 | HR |
67 | 15208 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | HR |
68 | 6034 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | HR |
69 | 16108 | 6.67 | 6.67 | 10.00 | 11.67 | 11.67 | 9.33 | R |
70 | 6000 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | HR |
71 | 15277 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | HR |
72 | 17328 | 3.56 | 3.56 | 10.69 | 10.69 | 10.69 | 7.84 | R |
73 | 17805 | 5.26 | 5.26 | 7.89 | 7.89 | 7.89 | 6.84 | R |
74 | 22847 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | HR |
75 | 16194 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | HR |
76 | 15388 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | HR |
77 | 1105 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | HR |
78 | 16659 | 6.90 | 6.90 | 12.14 | 12.14 | 12.14 | 10.05 | S |
79 | 15494 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | HR |
80 | 16161 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | HR |
81 | 17126 | 1.85 | 1.85 | 5.56 | 5.56 | 5.56 | 4.07 | HR |
82 | 41767 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | HR |
83 | 1586 | 2.00 | 2.00 | 4.00 | 4.00 | 4.00 | 3.20 | HR |
84 | 15764 | 2.50 | 2.50 | 2.50 | 2.50 | 2.50 | 2.50 | HR |
85 | 16482 | 3.61 | 3.61 | 7.23 | 7.23 | 7.23 | 5.78 | R |
86 | 17406 | 4.22 | 4.22 | 7.67 | 10.17 | 10.17 | 7.29 | R |
87 | 40946 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | HR |
88 | 17094 | 3.45 | 5.17 | 5.17 | 6.90 | 6.90 | 5.52 | R |
89 | 16088 | 7.89 | 7.89 | 7.89 | 7.89 | 7.89 | 7.89 | R |
90 | 15439 | 14.67 | 14.67 | 15.33 | 15.33 | 15.33 | 14.07 | S |
91 | 15220 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | HR |
92 | 15611 | 6.67 | 6.67 | 8.33 | 10.00 | 10.00 | 8.33 | R |
93 | 15729 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | HR |
94 | 15211 | 0.00 | 0.00 | 2.27 | 2.27 | 2.27 | 1.36 | HR |
95 | 16168 | 8.23 | 8.23 | 11.51 | 11.51 | 11.51 | 10.19 | S |
96 | Susceptible check – 77.89 (7042S) | |||||||
Table - 2: Reaction of pearl millet germplasms against downy mildew disease based on disease incidence
| Sr. No. | Disease reaction | Scales (Incidence) | Number of germplasms | Name of germplasms |
1 | Highly resistant | 0-5 % | 61 | 16976, 16110, 42246, 18037, 16625, 15691, 41530, 15041, 17130, 17659, 15708, 15695, 20229, 22923, 15787, 1421, 5972, 17840, 5969, 15067, 16010, 20437, 18580, 1225, 42182, 42479, 1202, 16101, 42852, 21334, 16317, 18023, 22559, 22726, 41215, 17928, 17703, 17933, 17633, 6067, 16128, 17604, 6055, 15208, 6034, 6000, 15277, 22847, 16194, 15388, 1105, 15494, 16161, 17126, 41767, 1586, 15764, 40946, 15220, 15729, 1521 |
2 | Resistant | 5-10 % | 22 | 15078, 1220, 1206, 42299, 16745, 16322, 15743, 42078, 17479, 5984, 17179, 22827, 42063, 16060, 16108, 17328, 17805, 16482, 17406, 17094, 16088, 15611 |
3 | Susceptible | 10-25 % | 12 | 15805, 1342, 22822, 15924, 18839, 17734, 15738, 15001, 15325, 16659, 15439, 16168 |
4 | Highly susceptible | > 25 % | -Nil- | -Nil- |
Table – 3: Severity of downy mildew disease in pearl millet germplasms
Sr. No. | Germplasms | Downy mildew disease severity | Mean severity (%) | |||||||
30 DAS | 45 DAS | 60 DAS | 75 DAS | 90 DAS | ||||||
1 | 16976 | 3.03 | 3.03 | 3.41 | 3.41 | 3.41 | 3.26 | |||
2 | 16110 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | |||
3 | 42246 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | |||
4 | 18037 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | |||
5 | 15805 | 9.68 | 10.48 | 12.10 | 12.50 | 12.90 | 11.53 | |||
6 | 15078 | 6.25 | 6.25 | 6.88 | 6.88 | 6.88 | 6.63 | |||
7 | 22923 | 1.67 | 1.67 | 2.50 | 2.92 | 2.92 | 2.33 | |||
8 | 1220 | 1.56 | 3.13 | 4.69 | 4.69 | 4.69 | 3.75 | |||
9 | 20229 | 0.00 | 0.00 | 0.76 | 0.76 | 0.76 | 0.45 | |||
10 | 15787 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | |||
11 | 1421 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | |||
12 | 5972 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | |||
13 | 1342 | 7.26 | 7.26 | 8.06 | 8.47 | 8.87 | 7.98 | |||
14 | 17840 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | |||
15 | 18580 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | |||
16 | 1225 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | |||
17 | 22822 | 5.47 | 6.25 | 7.42 | 7.42 | 7.81 | 6.88 | |||
18 | 42182 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | |||
19 | 42479 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | |||
20 | 1202 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | |||
21 | 1206 | 6.00 | 6.00 | 7.50 | 7.50 | 7.50 | 6.90 | |||
22 | 5969 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | |||
23 | 15067 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | |||
24 | 16010 | 0.00 | 2.88 | 2.88 | 3.37 | 3.37 | 2.50 | |||
25 | 20437 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | |||
26 | 15695 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | |||
27 | 16625 | 2.42 | 2.42 | 3.23 | 3.23 | 3.23 | 2.90 | |||
28 | 42299 | 4.63 | 5.09 | 6.02 | 6.02 | 6.48 | 5.65 | |||
29 | 15691 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | |||
30 | 41530 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | |||
31 | 15041 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | |||
32 | 16745 | 3.70 | 3.70 | 4.17 | 4.63 | 4.63 | 4.17 | |||
33 | 16322 | 7.81 | 7.81 | 8.98 | 8.98 | 8.98 | 8.52 | |||
34 | 17130 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | |||
35 | 17659 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | |||
36 | 15924 | 10.71 | 10.71 | 11.61 | 11.61 | 12.05 | 11.34 | |||
37 | 15743 | 3.13 | 3.13 | 4.69 | 4.69 | 4.69 | 4.06 | |||
38 | 18839 | 11.84 | 11.84 | 15.13 | 15.13 | 15.13 | 13.82 | |||
39 | 15708 | 1.43 | 1.43 | 2.86 | 2.86 | 2.86 | 2.29 | |||
40 | 16101 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | |||
41 | 42852 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | |||
42 | 17734 | 11.25 | 11.25 | 12.92 | 12.92 | 12.92 | 12.25 | |||
43 | 21334 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | |||
44 | 16317 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | |||
45 | 42078 | 4.30 | 5.08 | 5.08 | 5.47 | 5.47 | 5.08 | |||
46 | 18023 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | |||
47 | 22559 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | |||
48 | 17479 | 4.84 | 4.84 | 5.24 | 5.24 | 5.24 | 5.08 | |||
49 | 15738 | 5.00 | 5.00 | 5.42 | 5.42 | 5.42 | 5.25 | |||
50 | 22726 | 1.56 | 1.56 | 1.56 | 1.56 | 1.56 | 1.56 | |||
51 | 41215 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | |||
52 | 17928 | 1.67 | 1.67 | 1.67 | 1.67 | 1.67 | 1.67 | |||
53 | 17703 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | |||
54 | 17933 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | |||
55 | 17633 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | |||
56 | 5984 | 3.33 | 3.33 | 5.83 | 5.83 | 5.83 | 4.83 | |||
57 | 6067 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | |||
58 | 17179 | 6.25 | 6.25 | 7.03 | 7.03 | 7.03 | 6.72 | |||
59 | 22827 | 6.90 | 6.90 | 6.90 | 6.90 | 6.90 | 6.90 | |||
60 | 16128 | 3.85 | 3.85 | 3.85 | 4.33 | 4.33 | 4.04 | |||
61 | 15001 | 10.16 | 10.16 | 11.33 | 12.50 | 12.50 | 11.33 | |||
62 | 42063 | 5.36 | 6.25 | 7.59 | 7.59 | 7.59 | 6.88 | |||
63 | 16060 | 4.17 | 4.17 | 4.17 | 4.17 | 4.17 | 4.17 | |||
64 | 15325 | 9.00 | 9.50 | 10.00 | 11.00 | 11.00 | 10.10 | |||
65 | 17604 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | |||
66 | 6055 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | |||
67 | 15208 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | |||
68 | 6034 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | |||
69 | 16108 | 6.67 | 6.67 | 8.33 | 8.75 | 8.75 | 7.83 | |||
70 | 6000 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | |||
71 | 15277 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | |||
72 | 17328 | 3.13 | 3.13 | 5.47 | 5.47 | 5.47 | 4.53 | |||
73 | 17805 | 2.94 | 2.94 | 3.31 | 3.31 | 3.31 | 3.16 | |||
74 | 22847 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | |||
75 | 16194 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | |||
76 | 15388 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | |||
77 | 1105 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | |||
78 | 16659 | 10.71 | 10.71 | 16.07 | 16.07 | 16.07 | 13.93 | |||
79 | 15494 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | |||
80 | 16161 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | |||
81 | 17126 | 1.85 | 1.85 | 2.78 | 2.78 | 3.24 | 2.50 | |||
82 | 41767 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | |||
83 | 1586 | 2.27 | 2.27 | 2.84 | 2.84 | 2.84 | 2.61 | |||
84 | 15764 | 1.56 | 1.56 | 1.56 | 1.56 | 1.56 | 1.56 | |||
85 | 16482 | 3.23 | 3.23 | 5.24 | 5.24 | 5.24 | 4.44 | |||
86 | 17406 | 3.45 | 3.45 | 4.31 | 5.60 | 6.03 | 4.57 | |||
87 | 40946 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | |||
88 | 17094 | 3.45 | 4.74 | 4.74 | 6.03 | 6.03 | 5.00 | |||
89 | 16088 | 5.00 | 5.00 | 5.00 | 5.00 | 5.00 | 5.00 | |||
90 | 15439 | 11.67 | 12.50 | 13.33 | 13.33 | 13.33 | 12.83 | |||
91 | 15220 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | |||
92 | 15611 | 6.67 | 6.67 | 7.08 | 7.92 | 7.92 | 7.25 | |||
93 | 15729 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | |||
94 | 15211 | 0.00 | 0.00 | 0.46 | 0.46 | 0.46 | 0.28 | |||
95 | 16168 | 7.50 | 7.50 | 8.75 | 8.75 | 8.75 | 8.25 | |||
96 | Susceptible check – 67.32 (7042S) | |||||||||
The pearl millet germplasms were evaluated in downy mildew sick field under infector rows systems to identify the downy mildew resistant lines. The susceptible downy mildew cultivar 7042S was utilized as infector row as well as indicator row to measure the progress and disease pressure in the screening trial. The observations were recorded at 30 days after sowing and then after 15 days interval up to harvesting. Among 95 germplasms, 61 germplasms found highly resistant (16976, 16110, 42246, 18037, 16625, 15691, 41530, 15041, 17130, 17659, 15708, 15695, 20229, 22923, 15787, 1421, 5972, 17840, 5969, 15067, 16010, 20437, 18580, 1225, 42182, 42479, 1202, 16101, 42852, 21334, 16317, 18023, 22559, 22726, 41215, 17928, 17703, 17933, 17633, 6067, 16128, 17604, 6055, 15208, 6034, 6000, 15277, 22847, 16194, 15388, 1105, 15494, 16161, 17126, 41767, 1586, 15764, 40946, 15220, 15729 and 1521), 22 germplasms found resistant (15078, 1220, 1206, 42299, 16745, 16322, 15743, 42078, 17479, 5984, 17179, 22827, 42063, 16060, 16108, 17328, 17805, 16482, 17406, 17094, 16088 and 15611), 12 germplasms found susceptible (15805, 1342, 22822, 15924, 18839, 17734, 15738, 15001, 15325, 16659, 15439 and 16168) and none of the germplasm was found highly susceptible (Table 1, 2 and 3).