Potential Use of Wild Legume Plant Exudates for the Prevention of Fusarium Wilt Disease ( Fusarium oxysporum ) in Chickpea (Cicer arietinum L.)

The aim of this study was to determine the potential of using wild legume exudates as a biocontrol agent against Fusarium oxysporium which causes wilt in chickpea plants. For this purpose, wild leguminous plants from Erzurum were collected and their exudates were obtained. From wild legume plants from which exudates were obtained in the study, belonging to the genus Onobrychis which It was determined that two of them are endemic and the other three are cosmopolitan species. Chickpea plant was grown in Green house and the plants were infected with F.oxysporium . Legume exudates were applied to the infected plants. The data like root stem length, were measured root stem length, chlorophyll, carotenoid


Introduction
Chickpea is a legume that has been produced and consumed in almost every part of the world since ancient times.Due to its protein content, rich in iron, calcium and other minerals, it is consumed as the main food source of people in developing countries.It is the third most cultivated agricultural plant in the world and Turkey ranks third after India and Pakistan in chickpea cultivation.With the changing climatic conditions and increasing population, the demand for human food and animal feed will increased.This result, in the importance of chickpeas in econmic value as a nurtient and its use as various the importance of legumes such as chickpeas which the economic value of chickpeas will increase in the near future ( Chickpea production is adversely affected by biotic and abiotic factors.Among factors fungal disease can cause econmic loss in chickpea production.Fungal pathogens prevent the growth and development of the plant by penetrating the vascular tissue of chickpea roots, restricting the transport of water and nutrients to the leaves.Fusarium wilt is one of the most important fungal diseases that cause serious losses in chickpea production.(Kumar et al. 2017;Suthar et al., 2017;Meshram et al., 2018).
Fusarium is the largest filamentous fungus genus common in agricultural areas that causes wilt disease (Khanzada et al., 2016).Many methods have been used to control plant pathogenic fungi.Fungicide applications have proven effective, but they are toxic to humans and other organisms.In addition, it is known that pathogenic fungi develop resistance to fungicides over time (Yoon et al., 2013;Pandey 2015).
Therefore, environmentally friendly approaches such as the use of plant exudates and extracts have been sought and used in recent years (Gurjar et al., 2012).In previous studies, it has been reported that plant extracts used against plant pathogenic diseases reduce the incidence of disease.Exudates vary considerably between agricultural plants and wild races.This difference gives wild breeds an advantage against pathogenic organisms and other abiotic stresses (Preece, and Peñuelas 2020).In line with this information, it was aimed to determine the potential of using wild legume exudates as a biocontrol agent against F. oxysporium, which causes wilt in chickpea plants.

Collection and Identification of Wild Legumes
Wild leguminous plants were collected from Erzurum province during the flowering period (June to July) and brought to the laboratory in sterile bags.The collected plants were identified.

Obtaining plant exudates
The plants were washed under the tap until the mud and soil were gone, and then washed 3 times with sterile distilled water.The plant samples were then placed in jars containing 1 liter of sterilized distilled water (20 plants in each jar) and sealed with sterile filter papers.These prepared containers were kept at room temperature for 21 days in a place out of direct light.At the end of the process, the plants were taken from the jars, the lids of the jars were closed with sterile lids, and the obtained exudates were kept at +4°C until used.

Growing chickpeas
Chickpea was used in the study Akçin 91; It was obtained from the Eastern Anatolia Agricultural Research Institute.The seeds were washed briefly with 96% alcohol and surface sterilized in 5% Sodium Hypochlorite for 5 minutes, then the seeds were rinsed three times with distilled water and allowed to swell for 5 hours in sterile distilled water.the swollen seeds were then germinated under sterile conditions and then planted in 1 L pots filled with sterilized sand.Germinated seedlings were grown on soil/vermiculite (3:1, v/v) at 25 °C in an environment-controlled chamber at a light intensity of 120 μmol photons m−2 s−1 and a 14/10 h light/dark photoperiod.

Development of F.oxysporum and its application to plants
F. oxysporium culture inoculated on Potato Dextrose Agar (PDA) was incubated at 28 C for 7 days.A prepared 5 ml spore suspension was then placed in 100 ml potato dextrose broth (PDB) in 250 Erlenmeyer and incubated at 28°C at 180 rpm for 96 hours.For each pot, 10 ml of mycelium was placed in 10 ml of isotonic water and the micelles were broken up with micelles.Then it was applied to the roots of 5-day old chickpea seedlings.24 hours after mycelial application, legume exudates were applied to seedling roots at a rate of 50 ml per pot.After the mycelial applications, the seedlings were irrigated with sterile tap water for 10 days.On day 15, the plants were harvested, root, stem lengths, and weights were taken, and the plant parts were stored at -18 °C for further analysis after lyophilization in liquid nitrogen.

Determination of lipid peroxidation and antioxidant enzyme activity
Lipid peroxidation was determined by Heath and Packer (1968) method, to determine the activities of antioxidant enzymes, fresh leaves (0.5 g) were ground with a mortar and pestle under chilled conditions in the presence of phosphate buffer (0.1 M, pH 7.5) containing 0.5 mM EDTA.The homogenate was centrifuged at 12,000 g for 10 min at 4 ˚C, and the resulting supernatant was used for the enzyme assay.SOD activity was assayed using the method of Agarwal and Pandey 2004.POX activity was measured according to the method of Zhang and Kirkham (1994).CAT activity was performed according to Qiu et al. (2011.)

Determination of photosynthetic pigments
Fresh leaf tissues (0.1 g) were homogenized in chilled 80 % (v/v) acetone.The homogenate was centrifuged at 8800 g for 10 min at 4 ˚C in dark.The absorbance of the acetone extract was measured at 663, 645, and 470 nm using a spectrometer (Shimadzu UV mini-1240).The contents of chlorophyll a, chlorophyll b, and total carotenoids were calculated according to Arnon 1949.

Statistical analysis
All experiments were performed 6 times and the average of values was presented.The data were analyzed by analysis of variance, and means were compared by using Duncan's Multiple Range Test at p < 0.05 significance level.

Results
In the study, the plants obtained from exudates and used against F.oxysporium were diagnosed in the Department of Biology of Atatürk University.
According to the diagnostic results, it was determined that two of the plants from which exudates were obtained were endemic and the other three were cosmopolitan species (Table 1).

Effect on Lipid peroksidasyon (LPO)
In F.oxysporium application, the amount of LPO increased in both root and stem.In almost all exudate applications, LPO levels close to the control were measured.This shows that the applied exudates are an effective agent against wilt (Table 4).

Effects on Enzyme Activity
In the study, antioxidant enzymes in the root and stem were examined separately.Although CAT enzyme activity was higher in F.oxysporium application compared to control, it was observed that it could not cope with wilt, which is the abiotic stress factor.CAT enzyme activities increased in both root and stem in all exudate applications.However, the highest activity was observed in O3 (Onobrychis stenostochya) exudate application (Table 5).7).
).In our study, the amount of chlorophyll in the chickpea plant exposed to the pathogen decreased significantly compared to the control.Chlorophyll and carotenoid amounts increased in plant exudate applications with F.oxysporium (Table 3).F.oxysporium, which is a pathogen in many plants, increases H2O2 reactive oxygen species and lipid peroxidation in plants.As it is known, ROS cause disruption of cellular metabolism and DNA damage.In addition, the increase in the amount of ROS is accepted as an indicator of the presence of stress factors in biological organisms.(Kunstler et al. 2015;Camejo et al. 2016;Sewelam et al. 2016).Plants, like many organisms, have defense mechanisms to cope with these stress factors.Catalase (CAT) and peroxidase (POX) enzyme and superoxide enzyme that breaks down superoxide anion are the main elements of this defense mechanism (Jayamohan et al. 2018).Fusarium wilt is one of the major biotic stresses that reduce chickpea yield.In this study, F.oxysporium applied to the chickpea plant caused stress and significantly increased the LPO amount in both root and stem compared to the control.The plant extracts we use to combat pathogens have somewhat reduced the amount of LPO.especially F.oxysporium+O3 and F.oxysporium+O4 applications brought this rate to the level of the control group.(Table1).Considering the LPO amounts, the antioxidant enzyme system elements SOD, CAT and Pox amounts; There is a significant increase in the amount of F.oxysporium applied plants and plants in the control group (Tables 5, 6 and 7).The applied plant exudates contributed to the defense system of the chickpea plant with the primary and secondary metabolites they contained and gained resistance against F.oxysporium.Were et al. ( 2021), in their study, reported that they inhibited the development of F.oxysporim by using the exudates of 2 types of leguminous plants, and they determined that this feature was the phenolic substances in the exudates.Many  ).This study coincides with the literature information.In addition, each plant exudate had different effects.In this study, the antifungal effect of wild legume strains against F. oxysporium was evaluated and it was seen that the endemic species O3 and O4 were more effective.This is thought to be due to the fact that they are grown in different regions and their contents are different (Table4,5,6and 7).
As a result, it was concluded that the exudates obtained from wild legume plants can be used against F.oxysporium and as a non-toxic and economically suitable fungicidal.In addition to a preliminary study used against F.oxysporim, which is a pathogen in chickpea and many plants, of the exudates of both endemic species and cosmopolitan species of Onobrychis legumes.

Conclusions
In this study, it was determined by evaluating the morphological and physiological parameters that the exudates of Onobrychis species can be used as a natural and effective biocontrol agent against F. oxysporium.

Table 1 :
Scientific names, distribution and codes of the plants from which exudates are obtained

Table 2 :
Root length, stem length, fresh weight, and plant dry matter of chickpea after treatments with Fusarium wilt disease and wild legume plant exudates

Table 3 :
Chlorophyll and Carotinoide contents

Table 4 :
Lipid peroxidation (LPO) of roots and stems of chickpea after treatments with Fusarium wilt disease and wild legume plant exudates

Table 5 :
CAT enzyme activity

Table 6 :
SOD enzyme activity F:oxysporum application (Table